EFFECT OF FEEDING ON DISTRIBUTION OF FATTY-ACIDS AT SN-2-POSITION IN TRIACYLGLYCEROLS OF DIFFERENT ADIPOSE TISSUES IN LAMBS

Studies on the fatty acid composition and the positional distribution of fatty acids within the triacylglycerols have been made in male lamb s. The effect of feeding level was studied on fatty acid composition a nd distribution in triacylglycerols (TG) of subcutaneous (SAT), breast (BAT) and perirenal (PAT) adipose tissue. Different feeding levels ex erted no significant effect on fatty acids (except 18:2) and positiona l distribution of fatty acids (FA) at Sn-2-position in TG of BAT. Rega rdless of smaller quantity of unsaturated FA in PAT, these acids were esterified with greater specificity at the second position, compared t o BAT. With increasing energy in the diet, the coefficients of distrib ution for 18:1 and 18:2 reduced significantly and leveled with those i n BAT. Subcutaneous adipose tissue was characterized by parallel incre ases of relative quantity of 18:1 in TG molecules and in Sn-2-position for groups on the highest energy diet. In all three adipose tissues t he high energy density of ration lead to significant increases of rela tive 18:2 contents. Results show that the relative quantities of FA an d specificity of their distribution in TG molecules depend on feeding level and are characteristic for different adipose tissues. Changes of 18:1, 18:2 and 18:0 contents, with higher energy supply, made lipids of perirenal and subcutaneous adipose tissue less saturated.