We reexamine the problem of a hole moving in an antiferromagnetic spin
background and find that the injected hole will always pick up a sequ
ence of nontrivial phases from the spin degrees of freedom. Previously
unnoticed, such a stringlike phase originates from the hidden Marshal
l signs which are scrambled by the hopping of the hole. We can rigorou
sly show that this phase string is nonrepairable at low energy and giv
e a general proof that the spectral weight Z must vanish at the ground
-state energy due to the phase-string effect. Thus, the quasiparticle
description fails here and the quantum interference effect of the phas
e string dramatically affects the long-distance behavior of the inject
ed hole. We introduce a so-called phase-string formulation of the t-J
model for a general number of holes in which the phase-string effect c
an be explicitly tracked. As an example, by applying this new mathemat
ical formulation in one dimension, we reproduce the well-known Lutting
er-liquid behaviors of the asymptotic single-electron Green's function
and the spin-spin correlation function. We can also use the present p
hase-string theory to justify previously developed spin-charge separat
ion theory in two dimensions, which offers a systematic explanation fo
r the transport and magnetic anomalies in the high-T-c cuprates.