A METHOD FOR SIMULTANEOUS MEASUREMENT OF INTRAGASTRIC, INTRADUODENAL,AND INTRACOLONIC PRESSURES IN THE BILATERALLY VAGOTOMIZED SPINAL RAT

A method has been developed where quantitative evaluation of intragast ric (IGP), intraduodenal (IDP), and intracolonic (ICP) pressures (cm H 2O) can be obtained in the bilaterally vagotomized spinal rat. The pre paration is very sensitive to 5-hydroxytryptamine (5-HT) and 5-HT-agon ists, carbachol, histamine, dopamine, and/or noradrenaline. The effect s of drugs on IGP, IDP, and ICP (increase or decrease) can be assessed and a dose-response curve before and after antagonists can be compute d. The described experimental model is simple, reliable, reproducible, and appropriate to study and differentiate the different gastrointest inal system receptors that seem to be involved in the modulation of ga strointestinal motility. The additional advantage in using this method is that drug effects on the motility of stomach, duodenum, and colon can be investigated simultaneously.