ADRENERGIC-LIPOLYSIS IN IMMOBILIZED AND PERFUSED ADIPOCYTES

The method of cellular immobilization and perfusion was applied to adi pocytes. The lipolytic effect of isoprenaline, whose action is produce d as a result of receptor-drug interaction, was followed. An agarose s olution kept at at 37 degrees C was mixed 1:1 with the cell suspension . Thereafter, adipocytes were immobilized in the agarose threads. The lipolytic effect of 0.1 mi of isoprenaline (1x10(-4) mol/l), that was rapidly introduced to the cell perfusion inlet in a non-recirculating system, was monitored by assessing glycerol production. The immobilize d and perfused adipocytes exhibited significant lipolytic activity. Af ter reaching the maximum effect, 0.1 mi of propranol (1x10(-3) mol/l) that was applied to the bioreactor inlet, abolished the isoprenaline e ffect. The present data demonstrate the potential applicability of imm obilized perfused adipocytes For various kinds of studies.