IN-VIVO STUDY OF THE ANTILIPOPEROXIDANT E FFECT OF 3',5,7-TRIHYDROXY-4'-METHOXY FLAVONE-7-RUTINOSIDE

In 3-month-old Wistar rats carrageenan and CCl4 injected intraperitone aly induce an acute phase reaction which is characterized by a marked increase in alpha 1, alpha 2, beta serum globulins. This reaction corr esponds to a large increase in these globulins in the first case and a smaller one in the second. A lipoperoxidant effect is demonstrated by the serum lipoprotein mobility as the lipoperoxidation index (in MDA units) or the decrease in serum vitamin A and E concentrations. This e ffect is also greater in the first case than in the second one. In the same way the lipoperoxidant effect is shown in liver microsomes' but with a lower amplitude in the fil st case than in the second one. The treatment of rats by intraperitoneal injection of diosmine (150 mg/kg per week) during the 8 weeks which precede the injection of carrageena n or CC14 results in : i) a marked decrease in the acute-phase reactio n and a lower one in the lipoperoxidant effect, in serum; ii) a den ea se in the CCl4 induced lipoperoxidant effect in liver microsomes. It m ay be concluded that diosmine, not injected at the same time as carrag eenan or CCl4, but during the previous 8 weeks is sufficiently well di stributed in the whole body to produce a marked inhibition of the acut e phase reaction and a perceptible effect on lipoperoxidation. It may be considered an effective complement to the natural antioxidant defen ces of the organism (vitamins A and E).