ISOLATION OF PERITONEAL PRECURSORS OF B-1 CELLS IN THE ADULT-MOUSE

Citation
Mar. Marcos et al., ISOLATION OF PERITONEAL PRECURSORS OF B-1 CELLS IN THE ADULT-MOUSE, European Journal of Immunology, 24(5), 1994, pp. 1033-1040
Citations number
50
Categorie Soggetti
Immunology
ISSN journal
00142980
Volume
24
Issue
5
Year of publication
1994
Pages
1033 - 1040
Database
ISI
SICI code
0014-2980(1994)24:5<1033:IOPPOB>2.0.ZU;2-I
Abstract
Two weeks of daily peritoneopheresis of adult mice result in the selec tive depletion of B-1 cells, followed by the appearance of a populatio n of B220(+)IgM(-) lymphocytes in the peritoneal cavity. These cells s hare with bone marrow (BM) pre-B cells expression of lambda 5, V-preB, and RAG-1 genes and a higher fraction of unrearranged V to DJ heavy ( H) chain immunoglobulin (Ig) gene segments,when compared with mature B lymphocytes. Upon transfer to SCID recipients, sorted peritoneal B220 (+)IgM(-) cells fail to colonize the BM, repopulate very few B cells i n the spleen, but entirely reconstitute the B-1 cell compartment in th e peritoneal and pleuropericardial cavities, In contrast, parallel tra nsfers of sorted BM B220(+)IgM(-) cells result in reconstitution of th e BM and spleen B lineage cell compartments, but in no coelomic B cell repopulation. Both types of pre-B cells reconstitute splenic plasma c ells of donor origin, but with markedly distinct efficiencies: the rat io of IgM-plasma cell/B cell numbers in the spleens of peritoneal pre- B cell recipients is more than 500-fold higher than that of recipients reconstituted by BM pre-B cells. We take these data to indicate that (1) differentiative commitment to the B-1 cell population occurs befor e selection events on mature cells; (2) B-1 precursors exist or may be locally produced in the adult mouse; (3) there is a lineage-related d ifferential ability of mature B cells to undergo terminal differentiat ion to high-rate Ig secretion.