CLONING OF GENOMIC LOCI AND CHROMOSOMAL LOCALIZATION OF THE HUMAN PCTAIRE-1 AND PCTAIRE-3 PROTEIN-KINASE GENES

Recent studies on the molecular mechanisms controlling the mammalian c ell cycle have disclosed a large family of cdc2-related serine/threoni ne kinases. Among this gene family, the PCTAIRE protein kinases compri se a distinct subfamily of unknown cellular function. To analyze the g enomic structure and chromosomal location of the PCTAlRE-1 and -3 gene s, we isolated human cosmid clones for each gene by screening a human genomic library with murine PCTAIRE cDNA probes. Overlapping clones en compassing approximately 60 kb of genomic DNA were obtained for both P CTLAIRE-1 and -3. These clones were confirmed to encode authentic PCTA IRE genes by the detection of exon-intron structures and the coinciden ce of the nucleotide sequence of exons to that of the published human cDNAs. Using these cosmid clones as probes for FISH analyses, the chro mosomal loci for PCTAIRE-1 and PCTAIRE-3 were assigned to bands Xp11 a nd 1q31-q32, respectively. (C) 1994 Academic Press, Inc.