ASSIGNMENT OF XERODERMA-PIGMENTOSUM GROUP-C (XPC) TO CHROMOSOME 3P25

The human gene XPC (formerly designated XPCC), which corrects the repa ir deficiency of xeroderma pigmentosum (XP) group C cells, was mapped to 3p25. A cDNA probe for Southern blot hybridization and diagnostic P CR analyses of hybrid clone panels informative for human chromosomes i n general and portions of chromosome 3 in particular produced the init ial results. Fluorescence in situ hybridization utilizing both a yeast artificial chromosome DNA containing the gene and XPC cDNA as probes provided verification and specific regional assignment. A conflicting assignment of XPC to chromosome 5 is discussed in Light of inadequacie s in the exclusive use of microcell-mediated chromosome transfer for g ene mapping. (C) 1994 Academic Press, Inc.