IDENTIFICATION OF A TXREB PSEUDOGENE (TXREBP) LOCATED BETWEEN THE GENES FOR P55 (MPP1) AND GBPD ON XQ28

A fibroblast cDNA library was screened by hybridization to a yeast art ificial chromosome containing genomic sequences from human Xq28. The m ajority of positive cDNA clones were found to correspond to the cDNA c oding for TXREB, an HTLV-1 enhancer-binding protein. Sequence analysis of the Xq28 genomic DNA revealed a number of deleterious changes comp ared to the previously reported cDNA In addition, both the genomic DNA and cDNA isolates were found to be lacking a 599-bp sequence, bracket ed by GT and AG, in the 5' untranslated region. These results suggest that the Xq28-linked gene is a processed pseudogene for TXREB and that the previously reported cDNA was only partially processed. Southern b lot analysis on a hybrid mapping panel confirmed the presence of at le ast one autosomal gene for TXREB, and Northern blot hybridization with the 599-bp putative intron probe confirmed that the sequence is not p art of the mature mRNA Further analysis showed that the gene is expres sed in a variety of human tissues and that the pseudogene is located b etween the genes for the proteins p55 and G6PD. (C) 1994 Academic Pres s, Inc.