METHIONINE-ENKEPHALIN IS HYDROLYZED BY AMINOPEPTIDASE-N ON CD4(-CELLS() AND CD8(+) SPLEEN T)

Authors
MILLER BC THIELE DL HERSH LB COTTAM GL
Citation
Bc. Miller et al., METHIONINE-ENKEPHALIN IS HYDROLYZED BY AMINOPEPTIDASE-N ON CD4(-CELLS() AND CD8(+) SPLEEN T), Archives of biochemistry and biophysics, 311(1), 1994, pp. 174-179
Citations number
30
Categorie Soggetti
Biology,Biophysics
Journal title
Archives of biochemistry and biophysics → ACNP
ISSN journal
00039861
Volume
311
Issue
1
Year of publication
1994
Pages
174 - 179
Database
ISI
SICI code
0003-9861(1994)311:1<174:MIHBAO>2.0.ZU;2-X
Abstract
Exogenous methionine enkephalin incubated with CD4+ or CD8+ T cells pu rified from murine spleen is metabolized primarily, if not exclusively , by aminopeptidase N (aminopeptidase M, EC 3.4.11.2), a membrane-anch ored ectopeptidase. The enzyme activity is identified by its substrate specificity, sensitivity to inhibition by amastatin, and immunoreacti vity with antibody to rat kidney aminopeptidase N. Activation of CD4T cells results in a small increase per cell in aminopeptidase N activ ity. (C) 1994 Academic Press, Inc.