CULTIVATION OF AN L-LACTATE DEHYDROGENASE MUTANT OF BACILLUS-STEAROTHERMOPHILUS IN CONTINUOUS-CULTURE WITH CELL RECYCLE

Authors
SANMARTIN R BUSHELL D LEAK DJ HARTLEY BS
Citation
R. Sanmartin et al., CULTIVATION OF AN L-LACTATE DEHYDROGENASE MUTANT OF BACILLUS-STEAROTHERMOPHILUS IN CONTINUOUS-CULTURE WITH CELL RECYCLE, Biotechnology and bioengineering, 44(1), 1994, pp. 21-28
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology and bioengineering → ACNP
ISSN journal
00063592
Volume
44
Issue
1
Year of publication
1994
Pages
21 - 28
Database
ISI
SICI code
0006-3592(1994)44:1<21:COALDM>2.0.ZU;2-F
Abstract
Continuous fermentation with cell recycle proved very effective in inc reasing the ethanol volumetric productivity of the thermophilic facult ative anaerobe, Bacillus stearothermophilus strain LLD-15, on sucrose at 70 degrees C. When complete cell recycle was used, cell viability d ecreased after a few residence times and sucrose consumption was reduc ed. Operation using a constant bleed rate resulted in greater stabilit y and higher ethanol volumetric productivities. A mathematical model b ased on maintenance energy requirements provided an adequate descripti on of the system. (C) 1994 John Wiley and Sons, Inc.