C. Toskulkao et al., NEPHROTOXIC EFFECTS OF STEVIOSIDE AND STEVIOL IN RAT RENAL CORTICAL SLICES, Journal of clinical biochemistry and nutrition, 16(2), 1994, pp. 123-131
Nephrotoxic effects of stevioside and steviol on p-aminohippurate (PAH
) accumulation in rat renal cortical tissue were demonstrated. Subcuta
neous administration of stevioside (1.5 g/kg body weight) to rats for
9h significantly (p<0.001) decreased the ability of excised renal cort
ex slices to accumulate PAH in vitro by 63.4%. Stevioside had no effec
t on lipid peroxidation in the plasma and renal cortical slices at 9h
after administration. Addition of either stevioside (6.25-100 mu M) or
steviol (1.56-100 mu M) to the incubation medium inhibited PAH accumu
lation in the rat renal cortical slices after a 30-min incubation. Thi
s inhibitory effect was dose dependent, and was at its maximum at 25 m
u M. Steviol was more potent than stevioside in inhibiting PAH uptake
by renal cortical slices. Stevioside and steviol at a concentration of
100 mu M had no effect on lipid peroxidation in rat renal cortical sl
ices incubated for 30 min in vitro. These results suggest that the nep
hrotoxic effects induced by stevioside and steviol in rats are not rel
ated to lipid peroxidation.