Js. Clegg et al., EXTENSIVE INTRACELLULAR TRANSLOCATIONS OF A MAJOR PROTEIN ACCOMPANY ANOXIA IN EMBRYOS OF ARTEMIA-FRANCISCANA, Experimental cell research, 212(1), 1994, pp. 77-83
Cells of encysted gastrula embryos of the crustacean Artemia francisca
na exhibit extraordinary stability during prolonged anoxia. We find th
at they contain an abundant protein (referred to as ''26-kDa protein''
) that undergoes translocation to the nucleus during anoxia. The rever
se translocation rapidly occurs when anoxic embryos are returned to ae
robic conditions. A similar translocation appears to take place in emb
ryos exposed to 42 degrees C aerobic heat shock and prolonged exposure
to low temperature (0-2 degrees C), and in diapause embryos. Gel filt
ration and Western immunoblotting indicate that the 26-kDa protein is
translocated to other cellular compartments and may also be associated
with a wide variety of ''soluble'' proteins during anoxia. This prote
in makes up roughly 15% of the total nonyolk embryo protein and is, by
far, most abundant in the encysted embryo stage of the life cycle. Th
e hypothesis is advanced that the 26-kDa protein may play the role of
a metabolic regulator and/or a protective molecular chaperone during p
rolonged anoxia and other forms of stress. (C) 1994 Academic Press, In
c.