P. Heidrich et M. Wink, TAWNY OWL (STRIX-ALUCO) AND HUMES TAWNY OWL (STRIX-BUTLERI) ARE DISTINCT SPECIES - EVIDENCE FROM NUCLEOTIDE-SEQUENCES OF THE CYTOCHROME-B GENE, Zeitschrift fur Naturforschung. C, A journal of biosciences, 49(3-4), 1994, pp. 230-234
The cytochrome b gene of the Tawny Owl (Strix aluco), Hume's Tawny Owl
(Strix butleri) and the African wood owl (Strix woodfordii) was ampli
fied by polymerase chain reaction (PCR) and partially sequenced (300 b
ase pairs). Sequences differ substantially (9 to 12% nucleotide substi
tutions) between these taxa indicating that they represent distinct sp
ecies, which is also implicated from morphological and biogeographic d
ifferences. Using cytochrome b sequences of S. aluco, S. butleri, S. w
oodfordii, Athene noctua and Tyto alba phylogenetic relationship were
reconstructed using the ''maximum parsimony'' principle (PAUP 3.1.1) a
nd the neighbour-joining method (MEGA).