P. Zamorano et al., RECOGNITION OF B-CELL AND T-CELL EPITOPES BY CATTLE IMMUNIZED WITH A SYNTHETIC PEPTIDE-CONTAINING THE MAJOR IMMUNOGENIC SITE OF VP1 FMDV 01-CAMPOS, Virology, 201(2), 1994, pp. 383-387
The precise location of B and T cell epitopes have been established in
a peptide containing the major immunogenic site (residues 135-160) of
FMDV strain 01 Campos (01C) VP1. The peptide (p135-160), administered
free or conjugated to bovine serum albumin, induced complete protecti
on in guinea pigs and a strong neutralizing antibody (NAb) response in
cattle. Using a set of partially overlapping peptides it was shown th
at although several a cell epitopes were distributed along the p135-16
0, the residues responsible for the induction of NAb were restricted t
o the amino acids 135-144. In addition, bovines immunized with the p13
5-160 showed a strong proliferative response to this peptide but a ver
y poor response against purified virus. Two T-epitopes were located in
the 135-160 sequence: one on the region 135-144 and the other in the
region 150-160. The immunodominance of these two T cell epitopes was c
onfirmed in cattle immunized with inactivated virus vaccines. (C) 1994
Academic Press, Inc.