Seagrass biomass was estimated in a 37.649 km2 area in Moreton Bay, Qu
eensland. The study area was stratified by hand-digitising seven strat
a that were identified by photo-interpretation of a colour aerial phot
ograph. A pilot study was undertaken to calculate how many samples sho
uld be taken at each site and how best to allocate sampling effort to
the seven strata. Seagrass samples taken with a modified 'orange-peel'
grab and by coring were the same (P > 0.5). As the grab is easier to
use, quicker, can be operated by one person from a small dinghy, and d
oes not call for diving or wading, we used it for the main study. Four
seagrasses were found in the study area. The dominant seagrass was Zo
stera capricorni Aschchers., with smaller quantities of Halophila oval
is (R.Br.) Hook.f., Halophila spinulosa (R.Br.) Aschers. and Halodule
uninervis (Forssk.) Aschers. The total seagrass biomass in the study a
rea was estimated at 2145 +/- 568 t (95% confidence interval). Stratif
ication improved the precision of the simple random sampling estimate
by 68%. The use of a Geographic Information System (GIS) and Computer
Aided Drafting (CAD) for sample design, a Global Positioning System (G
PS) for locating sample sites in the field and the grab for taking sam
ples substantially enhanced research productivity and accuracy. The ex
perimental design is statistically robust and provides large samples f
or cost-effective and reliable estimates of seagrass biomass.