L. Plhak et P. Sporns, DEVELOPMENT AND PRODUCTION OF MONOCLONAL-ANTIBODIES FOR THE MEASUREMENT OF SOLANIDINE POTATO GLYCOALKALOIDS, American potato journal, 71(5), 1994, pp. 297-313
Although it is generally acknowledged solanidine glycoalkaloids found
in domestic potato cultivars are of sufficient toxicity to require tes
ting prior to the release of new varieties, the widespread use of one
of the most promising analysis techniques, immunoassays, is hampered b
y the variability of polyclonal antibody that has been developed for t
hese analyses. We report the development of a uniform monoclonal antib
ody (mouse IgG1) that binds to solanidine and demissidine alkaloids fo
r use in immunoassays. The monoclonal producing hybridoma developed is
sufficiently resilient to produce the antibody in protein free media,
from which very pure antibody could be isolated by simple ammonium su
lfate precipitation. The purified antibody can be freeze-dried for sto
rage and performs well in immunoassays with very low background absorb
ances. The monoclonal antibody was used in the optimized immunoassay a
t a concentration of 200 ng/mL with only 10 ng/mL concentration requir
ed for detectable signals (titer). From half absorbance levels (I50 va
lues) the antibody detected alpha-solanine the best (0.021 muM) while
alpha-chaconine, demissidine and solanidine were detected at levels of
0.043, 0.092 and 0. 103 muM, respectively.