RATIONALE AND OBJECTIVES. Indium-(111) (In-111)-labeled leukocyte scan
ning has been used frequently in patients suspected of having infectio
ns. Recently, technetium-99m hexamethylpropylene amine oxime ((TC)-T-9
9m-HMPAO) has been used to label leukocytes. This study was undertaken
to determine the distribution and dosimetry of Tc-99m-HMPAO leukocyte
s in healthy subjects. METHODS. Five healthy volunteers had leukocytes
labeled with Tc-99m-RMPAO. After injection of the labeled leukocytes,
whole body images and blood and urine samples were obtained at multip
le time points. RESULTS. Visual interpretation of the images demonstra
ted significant bowel activity as early as 2 hours and increasing with
time such that on the 8- and 24-hour images, the amount of bowel acti
vity would preclude using Tc-99m-HMPAO leukocyte scanning for abdomina
l processes. The dosimetry for this study is similar to that of other
studies and is in an acceptable diagnostic range. CONCLUSIONS. Tc-99m-
HMPAO leukocyte studies are an acceptable alternative to In-111-labele
d leukocyte studies in terms of dosimetry. Use of such studies in the
abdomen should be limited to early images, usually before 2 hours, to
avoid confusion with the normal route of excretion in the bowel.