5'-S-NUCLEOSIDE TRIPHOSPHATES - SYNTHESIS AND SUBSTRATE PROPERTIES INTESTS WITH DNA-POLYMERASES

In the course of our investigations on DNA polymerases a synthesis of new triphoshate analogues, 5'-mercapto-5'-deoxythymidine 5'-S-triphosp hate and 5'-mercapto-3',5'-dideoxythymidine 5'-S-triphosphate, was bro ught about starting from 3'-O-acetyl-thymidine and 3'-deoxythymidine, respectively. The former compound proved to be a weak substrate for E. coli DNA polymerase I and AMV reverse transcriptase and inactive towa rds DNA polymerases alpha from placenta and beta from rat liver. The s econd synthesized triphosphate was incorporated into DNA chain by none of the tested enzymes. Probable reasons for so strong a decrease in t he substrate properties of the prepared compounds are discussed.