IMAGE-ANALYSIS OF SEPARATION PROCESSES .1. DEVELOPMENT OF A VIDEO IMAGE ANALYZING SYSTEM AND ITS FUNDAMENTAL APPLICATION TO THE DIRECT OBSERVATION OF A SEPARATION PROCESS IN A COLUMN

In order to analyze the separation processes taking place in liquid ch romatography, a video image analyzing system was developed. A video ca mera, video tape recorder and an image processor were linked to a micr ocomputer. The distribution of fluorescent solutes in a glass column w as measured as a densitogram which was regarded as a chromatogram insi de the column. The apparent migration rate, which was the migration di stance for a unit migration volume, was determined by this method and was found to have a good linear relationship with the capacity factor k'. Band broadening during separation was also estimated at various fl ow rates. It was concluded that the band broadening was mainly due to multiple-path diffusion.