BRYONOLIC ACID PRODUCTION IN HAIRY ROOTS OF TRICHOSANTHES-KIRILOWII MAX VAR JAPONICA KITAM TRANSFORMED WITH AGROBACTERIUM-RHIZOGENES AND ITS CYTOTOXIC ACTIVITY

Authors
TAKEDA T KONDO T MIZUKAMI H OGIHARA Y
Citation
T. Takeda et al., BRYONOLIC ACID PRODUCTION IN HAIRY ROOTS OF TRICHOSANTHES-KIRILOWII MAX VAR JAPONICA KITAM TRANSFORMED WITH AGROBACTERIUM-RHIZOGENES AND ITS CYTOTOXIC ACTIVITY, Chemical and Pharmaceutical Bulletin, 42(3), 1994, pp. 730-732
Citations number
20
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
Journal title
Chemical and Pharmaceutical Bulletin → ACNP
ISSN journal
00092363
Volume
42
Issue
3
Year of publication
1994
Pages
730 - 732
Database
ISI
SICI code
0009-2363(1994)42:3<730:BAPIHR>2.0.ZU;2-4
Abstract
The hairy roots of Trichosanthes kirilowii MAX. var. japonica KITAM. w ere induced by Agrobacterium rhizogenes (ATCC 15834) on sterile shoots . The axenic culture of hairy roots proliferated 30 to 60-fold based o n the initial fresh weight after three weeks of culture in Murashige & Skoog liquid media. Bryonolic acid as the main triterpenoid was isola ted in a high yield, together with chondrillasterol from the hairy roo ts of this plant. Bryonolic acid showed strong inhibition of the growt h of B-16 melanoma cells.