GENETIC-RECOMBINATION BY PROTOPLAST FUSION IN THE DEUTEROMYCETE BEAUVERIA-BASSIANA

Authors
PACCOLAMEIRELLES LD AZEVEDO JL
Citation
Ld. Paccolameirelles et Jl. Azevedo, GENETIC-RECOMBINATION BY PROTOPLAST FUSION IN THE DEUTEROMYCETE BEAUVERIA-BASSIANA, Brazilian journal of genetics, 17(1), 1994, pp. 15-18
Citations number
NO
Categorie Soggetti
Genetics & Heredity
Journal title
Brazilian journal of genetics
ISSN journal
01008455 → ACNP
Volume
17
Issue
1
Year of publication
1994
Pages
15 - 18
Database
ISI
SICI code
0100-8455(1994)17:1<15:GBPFIT>2.0.ZU;2-I
Abstract
Protoplasts of auxotrophic mutants of Beauveria bassiana were produced by treating germ tubes with lytic enzymes. Regeneration frequency was 33%. The fusogenic agent used was polyediylene glycol and the fusion products were highly unstable. The sectors which emerged from the fusi on products were analyzed and recombinants were obtained. The electrop horetic pattern for esterases of parental strains and recombinant sect ors was determined. The protoplast fusion technique proved to be a val uable tool for studies of genetics and breeding of B. bassiana.