PLASMID GENES PARTICIPATING IN MICROCIN-C 51 SYNTHESIS

Microcin C51 is an antibiotic with a wide application range produced b y Escherichia coli cells. Using insertions of transposon Tn5 a set of mutations was induced in the recombinant plasmid pAST, which determine s synthesis of microcin C51. The mutations were physically mapped. Com plementation analysis was performed for insertions and deletions in th e Mic+ plasmids pAST and pUHAB that lead to the absence of microcin or immunity to it. The analysis showed that at least three plasmid genes take part in microcin production and two genes determine immunity of producer cells to microcin. Functioning of the ompR gene product was n ecessary for synthesis of microcin C51.