CONSTRUCTION OF THE GENOMIC LIBRARY OF TH E CYANOBACTERIUM SYNECHOCYSTIS SP PCC-6803 IN A COSMID VECTOR

The gene library of the phototrophic cyanobacterium Synechocystis PCC6 803 was constructed in the cosmid vector Lorist6. About approximately 3.10(3) recombinant clones were obtained, of which 760 were selected f or further work. Each clone was kept in an individual well of a microt iter plate. The clone grids obtained were hybridized with two DNA prob es, which contained the ndhE and dfr genes of Synechocystis PCC6803. T wo groups of probe-overlapping cosmids (11 and 8 clones respectively), containing insertions averaging of 39 kb, were isolated. The capacity of the gene library is nine equivalents of the Synechocystis PCC6803 genome and its representativity exceeds 99%.