EFFICIENCY OF AMNIOCYTE CULTIVATION FOR P RENATAL DIAGNOSTIC OF CHROMOSOMA DISEASES

Cytogenetic studies of 150 cell samples were performed. The fetus kary otype was established in 121 cases. Efficiency of the analysis differe d significantly, depending on methods of cell cultivation and preparat ion of chromosome plates: for the in situ method it was 50%; for the t rypsin method, 82%; and for the pipette method, 99%. Analysis of 63 sa mples demonstrated that when the pipette method is used, the results a re available as early as within the first week of cultivation; this me thod is reliable for revealing karyotypic mosaicism in individual cell colonies, can be used successfully from the 16th to the 26th week of pregnancy, and provides a high level of G-staining in prometaphase chr omosomes. Comparison of the three methods of prenatal genetics provide s unambiguous evidence in favor of the pipette method.