KIH-201 - A NEW ENHANCER OF CHEMILUMINESCENCE IN THE LUMINOL-HYDROGENPEROXIDE-PEROXIDASE SYSTEM CHARACTERIZED BY A HIGH-PERFORMANCE LUMINOMETER

We developed new phenolic enhancers of chemiluminescence in the lumino l-hydrogen peroxide-horseradish peroxidase reaction. We designed, synt hesized, and tested three series of. phenolic enhancers as follows: 1) hydroxybenzylidenc-cyclopentenediones (KIH-201, KIH-203, TX-1123, and TX-1124), 2) 4-hydroxybenzylidene-malononitriles (TX-825, TX-1121, TX -1122, TX-1840 and SF-6847), and 3) 2, 4-dichlorophenol derivative (TX -816). Luminescence measurements were performed with a new high-perfor mance luminometer, which have a superior photon counting system (gate time: 10-100 ms) and an automatic injector (variable range: 10-190 mu l). We found that the arylidene-cyclopentenedione KIH-201 y3'-methoxy- benzylidene)-4-cyclopentene-1,3-dione] was an effective enhancer simil ar to that of p-iodophenol, known as the most strong enhancer of this chemiluminescent system. This KIH-201 will facilitate the luminol - hy drogen peroxide - horseradish peroxidase reaction