Hr. Berthoud et al., DISTRIBUTION AND STRUCTURE OF VAGAL AFFERENT INTRAGANGLIONIC LAMINAR ENDINGS (IGLES) IN THE RAT GASTROINTESTINAL-TRACT, Anatomy and embryology, 195(2), 1997, pp. 183-191
Intraganglionic laminar endings (IGLEs) are special terminal structure
s of vagal afferent fibers and have been demonstrated in the myenteric
plexus of esophagus and stomach. In order to quantitatively map their
presence and distribution over the entire gastrointestinal tract, inc
luding the small and large intestines, vagal afferents were anterograd
ely labeled in vivo by microinjections of the fluorescent carbocyanine
dye DiI into the left or right nodose ganglion of adult male rats. In
the most successfully labeled cases the highest density of IGLEs was
found in the stomach, with about half to one-third of the myenteric ga
nglia receiving at least one IGLE. The proportion of myenteric ganglia
innervated by IGLEs decreased in the small intestine; however,because
of its large surface area this gut segment was estimated to contain t
he highest total number of IGLEs. Both the cecum and colon also contai
ned significant numbers of IGLEs. In the stomach, this vagal afferent
innervation by IGLEs was more or less lateralized, with less than 20%
of labeled IGLEs found on the contralateral side with respect to the i
njection. The left/ventral vagus contributed a larger proportion of IG
LEs to the proximal duodenum, while the right/dorsal vagus contributed
a larger proportion of IGLEs to the distal duodenum and jejunum. Lase
r scanning confocal microscopy on select specimens revealed further st
ructural details. The parent axon typically formed two or more branche
s that flanked the ganglia laterally, and in turn produced numerous hi
ghly arborizing laminar terminal branches that covered one or both fla
t sides of the ganglion in a dome-like fashion. The similar distributi
on patterns and structural details suggest a uniform function for the
IGLEs throughout the gastrointestinal tract, but there is as yet no cl
ear proof for any of the hypothesized roles as specialized mechanosens
ors or local effector terminals.