M. Caserta et al., CONFORMATIONAL INFORMATION IN DNA - ITS ROLE IN THE INTERACTION WITH DNA TOPOISOMERASE-I AND NUCLEOSOMES, Journal of cellular biochemistry, 55(1), 1994, pp. 93-97
Information in DNA is not limited to sequence information. Both local
and global conformational parameters are pivotal to the interaction wi
th a number of relevant proteins. The function of the major components
of the transcription machinery (RNA polymerase II, DNA topoisomerase
I, nucleosomes, the TATA-binding factor) is dependent on the topologic
al status of the substrate DNA molecule. The topological requirements
and the conformational consensus that dictate the rules for localizati
on of nucleosomes and define the active sites for DNA topoisomerase I
have been established; the reaction of DNA topoisomerase I is regulate
d by a topological feedback mechanism. The integrating function of the
free energy of supercoiling in the transcription process and the regu
latory role of DNA topoisomerase I are discussed. (C) 1994 Wiley-Liss,
Inc.