A. Matsuura et al., CYCLOPHOSPHAMIDE-INDUCED TOLERANCE IN FULLY ALLOGENEIC HEART-TRANSPLANTATION IN MICE, Cellular immunology, 155(2), 1994, pp. 501-507
We have established a long-lasting heart allograft tolerance in H-2-in
compatible combinations by intravenous injection of 10(8) allogeneic s
pleen cells followed by the administration of 200 mg/kg of cyclophosph
amide (CP). When C57BL/10 mice (B10, H-2(b)) were primed iv with 1 X 1
0(8) viable spleen cells from naive BALB/c mice (H-2(d)) and treated i
p with 200 mg/kg CP 2 days later, BALB/c heart allografts were lasting
in the recipients for as long as 8 weeks. On the other hand, B10 mice
receiving either allogeneic spleen cells or CP rejected the heart all
ograft within 10 days after transplantation. A direct approach assessi
ng the donor-reactive T cells for frequency and reactivity showed that
the donor-reactive T cells were partially deleted and the rest were r
endered unresponsive. CP-induced tolerance may be applied for the indu
ction of antigen-specific tolerance to allogeneic heart transplantatio
n. (C) 1994 Academic Press, Inc.