Allergenic cross-reactivity among three Fusarium species (F. solani, F
. equiseti, and F. moniliforme) was determined by crossed immunoelectr
ophoresis (CIE), ELISA inhibition, and immunoprint-inhibition analysis
. Inhibiting immunoprint was used to assess the cross-reactivity of in
dividual allergenic proteins in culture filtrate (CF), separated on SD
S-PAGE. Dose-response inhibition of band 14 kDa in immunoprints of F.
solani CF by antigens of F. solani CF, F. equiseti CF, and F. monilifo
rme CF demonstrated significant cross-reactivity of allergenic protein
s on analysis of densitometric scans. CIE and ELISA inhibition using h
yperimmune sera raised in rabbits against F. solani CF antigen also de
monstrated shared antigenicity among Fusarium species. Collectively, t
his study revealed the presence of shared as well as unique antigenic
and allergenic determinants in F. solani CF, F. equiseti CF, and F. mo
niliforme CF antigens.