SYNTHESIS OF NEW FRAGMENTS OF VP1 PROTEIN OF THE A(22) FOOT-AND-MOUTH-DISEASE VIRUS - FRAGMENTS 134-139, 134-145, 140-145, 150-155, 150-159

Authors
KHALIKOV SK ALIEVA SV ASHUROV SG
Citation
Sk. Khalikov et al., SYNTHESIS OF NEW FRAGMENTS OF VP1 PROTEIN OF THE A(22) FOOT-AND-MOUTH-DISEASE VIRUS - FRAGMENTS 134-139, 134-145, 140-145, 150-155, 150-159, Bioorganiceskaa himia, 20(4), 1994, pp. 393-405
Citations number
17
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
20
Issue
4
Year of publication
1994
Pages
393 - 405
Database
ISI
SICI code
0132-3423(1994)20:4<393:SONFOV>2.0.ZU;2-O
Abstract
Fragments 134-145 and 150-159 of the antigenic-region of the VP1 prote in of the A(22) foot-and-mouth disease virus were synthesized by class ic methods of peptide chemistry with isobutyl chloroformate as a coupl ing reagent. After purification by HPLG and amino acid analysis, the f ree peptides Gly-Lys-Tyr-Ser-Ala-Gly-Gly-Leu-Gly-Arg-Arg-Gly-OH and H- Leu-Ala-Ala-Arg-Val-Ala-Lys-Gln-Leu-Pro-OH were conjugated with BSA by means of N,N-dicyclohexylcarbodiimide. The conjugates were used, with complete Freund adjuvant, for immunization on guinea pigs. The antibo dies formed had a virus neutralization activity.