THE CONSTRUCTION OF MIXED POLYMERS CONSIS TING OF DNA FRAGMENTS WITH CONSENSUS PROMOTER ELEMENTS AND NONNUCLEOTIDE SPACERS

Mixed oligomers, representing oligonucleotides connected with long non nucleotide spacers, have been synthesized using phosphoramidite chemis try. The oligonucleotide moieties of the mixed oligomers fully or part ially correspond in the structure to the consensus elements of -35 (TT GACA) and -10 (TATAATG) regions of prokaryotic promoters, The nonnucle otide spacers, approximating in size 17-membered DNA fragments, were s ynthesized using phosphoramidite derivatives of polyethylene glycol (P EG600), tetraethylene glycol or dodecanediol. It is shown that the oli gonucleotide moieties of the mixed oligomers can form <<normal>> DNA l ike antiparallel complementary complexes, being the substrates of T4 D NA ligase. To obtain the DNA-Like polymers with alternating natural an d nonnatural regions or cyclic structures, the enzymatic ligation of d ifferent complexes of the oligomers synthesized was studied.