X. Delamballerie et al., IMPROVED CURRENT METHODS FOR AMPLIFICATION OF DNA FROM ROUTINELY PROCESSED LIVER-TISSUE BY PCR, Journal of Clinical Pathology, 47(5), 1994, pp. 466-467
With both a classic DNA preparation protocol (including removal of par
affin wax and protein digestion) and a DNA extraction protocol with Ch
elex 100, the hepatitis B virus genome was searched for using the poly
merase chain reaction (PCR) in 30 samples of paraffin wax embedded liv
er tissue from patients with chronic hepatitis. The classic protocol w
as more sensitive than the rapid Chelex 100 procedure (10 v six positi
ve samples). A third protocol, including removal of paraffin wax, prot
ein digestion, and Chelex 100 treatment of the digestion solution befo
re PCR, was more sensitive than the others (16 positive samples). It i
s concluded that it could therefore be helpful for PCR analysis of par
affin wax embedded liver tissue.