EFFECT OF CENTRIFUGING SHELL VIALS AT 3,500XG ON DETECTION OF VIRUSESIN CLINICAL SPECIMENS

Citation
Hd. Engler et St. Selepak, EFFECT OF CENTRIFUGING SHELL VIALS AT 3,500XG ON DETECTION OF VIRUSESIN CLINICAL SPECIMENS, Journal of clinical microbiology, 32(6), 1994, pp. 1580-1582
Citations number
13
Categorie Soggetti
Microbiology
ISSN journal
00951137
Volume
32
Issue
6
Year of publication
1994
Pages
1580 - 1582
Database
ISI
SICI code
0095-1137(1994)32:6<1580:EOCSVA>2.0.ZU;2-S
Abstract
An increase in shell vial centrifugation force to 3,500 x g and a conc omitant reduction in spin time to 15 min did not decrease the sensitiv ity of detecting viruses in clinical specimens compared with the accep ted practice of using 700 x g for 40 min. No damage to the cell monola yer (ML) at the higher g force was observed. Toxicity to the ML is dec reased with the shorter spin, probably because of reduced time of cont act between the specimen and the ML.