Hd. Engler et St. Selepak, EFFECT OF CENTRIFUGING SHELL VIALS AT 3,500XG ON DETECTION OF VIRUSESIN CLINICAL SPECIMENS, Journal of clinical microbiology, 32(6), 1994, pp. 1580-1582
An increase in shell vial centrifugation force to 3,500 x g and a conc
omitant reduction in spin time to 15 min did not decrease the sensitiv
ity of detecting viruses in clinical specimens compared with the accep
ted practice of using 700 x g for 40 min. No damage to the cell monola
yer (ML) at the higher g force was observed. Toxicity to the ML is dec
reased with the shorter spin, probably because of reduced time of cont
act between the specimen and the ML.