ESTRAMUSTINE PHOSPHATE BUT NOT ESTRAMUSTINE INHIBITS THE INTERACTION OF MICROTUBULE-ASSOCIATED PROTEIN-2 (MAP2) WITH ACTIN-FILAMENTS

The effect of estramustine and estramustine phosphate (EP) on the inte raction of microtubule associated protein 2 (MAP2) with actin has been examined, We show that (a) neither estramustine nor EP influences act in polymerisation (b) EP, but not estramustine, reduces the amount of MAP?. which cosediments with F-actin in a dose-dependent manner and (c ) EP decreases the MAP2-induced crosslinking of F-actin into gelled ne tworks. The data suggest, that unlike estramustine, EP interacts with MAP2 and modifies its interaction not only with microtubules but also with actin filaments. (C) 1997 Federation of European Biochemical Soci eties.