FLUOROGENIC SUBSTRATES CONTAINING 7-AMINO-4-METHYL-2-QUINOLINONE FOR AMINOPEPTIDASE-M, CHYMOTRYPSIN, ELASTASE AND TRYPSIN, DETERMINATION OFENZYME-ACTIVITY

Several new fluorogenic substrates for four proteases were synthesized by coupling the highly fluorescent 7-amino-4-methyl-2-quinolinone (AM eq) with appropriate amino acids and peptides. Compounds HCl . H-Ala-N H-Meq (1) and HCl . H-Leu-NH-Meq (2) are substrates for aminopeptidase M, Glt-Tyr-NH-Meq (3) and Glt-Leu-Tyr-NH-Meq (4) for chymotrypsin, Bo c-(Ala)4-NH-Meq (5) for elastase and Z-Arg-NH-Meq (6) for trypsin. The kinetic parameters for the hydrolysis of the new substrates were dete rmined and showed that each new substrate is suitable for a convenient fluorometric assay of the corresponding enzyme.