PLUMBAGIN, A NATURALLY-OCCURRING NAPHTHOQUINONE - ITS ISOLATION, SPECTROPHOTOMETRIC DETERMINATION IN ROOTS, STEMS, AND LEAVES IN PLUMBAGO-EUROPAEA L

Authors
ALNURI MA HANNOUN MA ZATAR NA ABUEID MA ALJONDI WJ HUSSEIN AI ALISHTAYEH MS
Citation
Ma. Alnuri et al., PLUMBAGIN, A NATURALLY-OCCURRING NAPHTHOQUINONE - ITS ISOLATION, SPECTROPHOTOMETRIC DETERMINATION IN ROOTS, STEMS, AND LEAVES IN PLUMBAGO-EUROPAEA L, Spectroscopy letters, 27(4), 1994, pp. 409-416
Citations number
9
Categorie Soggetti
Spectroscopy
Journal title
Spectroscopy letters → ACNP
ISSN journal
00387010
Volume
27
Issue
4
Year of publication
1994
Pages
409 - 416
Database
ISI
SICI code
0038-7010(1994)27:4<409:PANN-I>2.0.ZU;2-V
Abstract
A new method for isolation and spectrophotometric determination of plu mbagin is presented. Plumbagin was isolated by thin layer chromatograp hy (TLC) and column chromatography (CC) techniques, as an orange tinge d yellow long crystalline substances. Plumbagin exhibits two absroptio n maxima at 410 and 510 nm. Stability of the color, pK(a) value, and t he effect of pH were studied. Beer's law is obeyed over the range 0.9 - 45 ppm. The method is applied to the determination of plumbagin in r oots, stems, and leaves of Plumbago europaea L. plant.