[3-C-13] GAMMA-LINOLENIC ACID - A NEW PROBE FOR C-13 NUCLEAR-MAGNETIC-RESONANCE STUDIES OF ARACHIDONIC-ACID SYNTHESIS IN THE SUCKLING RAT

Our objective was to develop a suitable probe to study metabolism of p olyunsaturated fatty acids by C-13 nuclear magnetic resonance (NMR) in the suckling rat pup. [3-C-13] gamma-Linolenic acid was chemically sy nthesized, and a 20 mg (Experiment 1) or 5 mg (Experiment 2) dose was injected into the stomachs of 6-10-day-old suckling rat pups that were then killed over a 192 h (8 d) lime course. C-13 NMR showed that C-13 in gamma-linolenate peaked in liver total lipids by 12-h post-dosing and that [5-C-13]-arachidonic acid peaked in both brain and liver tota l lipids 48-96 h post-dosing. C-13 enrichment in brain gamma-linolenic acid was not detected by NMR, but gas chromatography-combustion-isoto pe ratio mass spectrometry showed that its mass enrichment in brain ph ospholipids at 48-96 h postdosing was 1-2% of that in brain arachidoni c acid: C-13 was present in liver and brain cholesterol and in perchlo ric acid-extractable water-soluble metabolites in the brain, liver and carcass. We conclude that low but measurable amounts of exogenous gam ma-linolenic acid do access the suckling rat brain in vivo. The slow t ime course of [5-C-13] arachidonic acid appearance in the brain sugges ts most of it was probably transported there after synthesis elsewhere , probably in the liver. Some carbon from gamma-linolenic acid is also incorporated into lipid products other than n-6 long-chain polyunsatu rated fatty acids.