Single-shell carbon nanotubes, approximately 1 nm in diameter, have be
en imaged for the first time by atomic force microscopy operating in b
oth the contact and tapping modes. For the contact mode, the height of
the imaged nanotubes has been calibrated using the atomic steps of th
e silicon substrate on which the nanotubes were deposited. For the tap
ping mode, the calibration was performed using an industry-standard gr
ating. The paper discusses substrate and sample preparation methods fo
r the characterization by scanning probe microscopy of nanotubes depos
ited on a substrate.