STANDARDIZED PERIPHERAL-BLOOD MONONUCLEAR CELL-CULTURE ASSAY FOR ZIDOVUDINE SUSCEPTIBILITY TESTING OF CLINICAL HUMAN-IMMUNODEFICIENCY-VIRUSTYPE-1 ISOLATES - EFFECT OF REDUCING THE NUMBERS OF REPLICATES AND CONCENTRATIONS

Authors
MARSCHNER IC MAYERS DL ERICE A SMEATON L JOHNSON VA RICHMAN DD REICHELDERFER P JAPOUR AJ
Citation
Ic. Marschner et al., STANDARDIZED PERIPHERAL-BLOOD MONONUCLEAR CELL-CULTURE ASSAY FOR ZIDOVUDINE SUSCEPTIBILITY TESTING OF CLINICAL HUMAN-IMMUNODEFICIENCY-VIRUSTYPE-1 ISOLATES - EFFECT OF REDUCING THE NUMBERS OF REPLICATES AND CONCENTRATIONS, Journal of clinical microbiology, 35(3), 1997, pp. 756-758
Citations number
5
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
3
Year of publication
1997
Pages
756 - 758
Database
ISI
SICI code
0095-1137(1997)35:3<756:SPMCAF>2.0.ZU;2-Z
Abstract
Zidovudine susceptibility was assessed for 525 clinical human immunode ficiency virus type 1 isolates, before and after reducing the number o f replicates and zidovudine concentrations in the standardized consens us peripheral blood mononuclear cell culture assay. We conclude that o mitting the 0.001 mu M concentration and using duplicate rather than t riplicate wells are valid and cost-effective modifications of this exp ensive assay.