H. Goto et al., NUCLEOTIDE-SEQUENCE OF THE NUCLEOPROTEIN GENE OF THE RC-CENTER-DOT-HLSTRAIN OF RABIES VIRUS, A SEED STRAIN USED FOR ANIMAL VACCINE PRODUCTION IN JAPAN, Virus genes, 8(2), 1994, pp. 91-97
By using a phage vector (lambda ZAP II) and the mRNA extracted from IM
R-32 cells infected with the RC.HL strain of rabies virus, we construc
ted a cDNA library from which four nucleoprotein (N)-specific cDNA clo
nes were obtained by Southern blot hybridization. These clones contain
ed a cDNA insert of about 1.4 kb, in which the longest open reading fr
ame was the same length as that reported for the N cDNA of three fixed
strains, CVS, PV, and SAD B19. When the nucleotide and deduced amino
acid sequences were compared between the RC HL and the three strains,
homology was within the range of 91.5-91.8% and 95.1-96.0%, respective
ly. Of 183 nucleotides of the RC.HL N-cDNA that were not identical to
that of the corresponding site of at least one of the three strains, 4
1 were shared with the CVS strain, whereas only three were shared with
either of the other two strains. In the amino acid sequence, we found
29 residues that were not shared in common with all of the four strai
ns, 11 of which were the substitutions with radically different amino
acids that might cause conformational changes of the protein, and, in
addition, five of which were located in the region close to the C term
inus. The number of such amino acid substitutions between the RC.HL an
d CVS strains was smaller than that of the other three strains. These
results are not inconsistent with the presumption that the RC HL and C
VS strains originated from the same laboratory strain of the Pasteur v
iruses.