COPULATORY APPARATUS AND DEPOSITION OF MALE ACCESSORY SECRETION IN PHORMIA-REGINA (DIPTERA, CALLIPHORIDAE)

A component of the male accessory gland secretion, injected during mat ing, was responsible for onset of refractory behavior in the female bl ack blow fly, Phormia regina (Meigen). Immediately after mating, two c omponents of the semen were located separately in the bursa copulatrix : sperm were found anteriorly near the opening to the spermathecal duc ts, and accessory secretion distended the posterior of the bursa. The accessory secretion disappeared within a day of mating, presumably ent ering the hemolymph from where it was detected. Scanning electron micr oscopy of the intromittent organ and phallosome, and reconstruction of the female reproductive tract from serial sections revealed complemen tary structures in the male and female. A pair of conspicuous flaps on the phallosome clasp a cuticular tonsil in the female, sealing the ac cessory secretion in the bursa during copulation. Accessory secretion is released at the tip of the phallosome and is redirected to a more p roximal release site on the phallosome via a pair of hitherto undescri bed accessory ducts, resulting in its deposition posteriorly in the bu rsa lumen.