EXPRESSION OF THE PVYO COAT PROTEIN (CP) UNDER THE CONTROL OF THE PVXCP GENE LEADER SEQUENCE - PROTECTION UNDER GREENHOUSE AND FIELD CONDITIONS AGAINST PVYO AND PVYN INFECTION IN 3 POTATO CULTIVARS
Kl. Hefferon et al., EXPRESSION OF THE PVYO COAT PROTEIN (CP) UNDER THE CONTROL OF THE PVXCP GENE LEADER SEQUENCE - PROTECTION UNDER GREENHOUSE AND FIELD CONDITIONS AGAINST PVYO AND PVYN INFECTION IN 3 POTATO CULTIVARS, Theoretical and Applied Genetics, 94(2), 1997, pp. 287-292
Coat protein-mediated resistance (CPMR), resistance conferred as a res
ult of the expression of viral coat proteins in transgenic plants, has
been illustrated to be an effective way of protecting plants against
several plant viruses. Nonetheless, consistent protection has not been
achieved for transgenic plants expressing the coat protein of potato
virus Y (PVY), the type member of the potyvirus family. In this report
, three different potato cultivars were transformed with a chimeric co
nstruct consisting of the capsid protein (CP) coding sequences of PVY
flanked by the AUG codon and the translational enhancer from the coat
protein gene of potato virus X (PVX). These cultivars were shown to ex
press high levels of PVY CP and confer a high degree of protection aga
inst PVYO and PVYN under both greenhouse and field conditions. In addi
tion, transgenic plants infected with potato virus A (PVA), a related
potyvirus, exhibited a delay in virus accumulation, which could be eas
ily overcome with increasing virus concentrations.