LARGE-PORE POLYDIMETHYLACRYLAMIDE RESIN FOR SOLID-PHASE PEPTIDE-SYNTHESIS - APPLICATIONS IN FMOC CHEMISTRY

We have synthesized a hydrophilic, cross-linked, aminoalkyl polydimeth ylacrylamide-beaded support upon which peptides have been assembled us ing standard Fmoc chemistry in automated batch-wise equipment. The res in was prepared by the free radical-initiated co-polymerization of N,N -dimethylacryl-amide, N,N'-bisacrylyl-1,3-diaminopropane and a functio nal monomer, N-methacrylyl-1,3-diaminopropane hydrochloride. After cou pling of N-alpha-tert-butyloxycarbonyl-glycine (Boc-glycine), amino ac id analyses gave resin loading capacities of 0.66 mmol/g. The resultin g polymer was highly swollen by polar solvents including aqueous buffe rs and had an exclusion limit of 50 kDa for soluble proteins. This res in was found to be an excellent support for peptide synthesis using Fm oc chemistry. Typical purities of crude peptides were 80%-95%, includi ng sequences that failed on conventional polystyrene resins.