G. Thedei et al., EFFECT OF CARBON SOURCE AND EXTRACELLULAR PH ON THE ACIDIFICATION OF THE CULTURE-MEDIUM AND PHOSPHATASE EXCRETION IN NEUROSPORA-CRASSA, Brazilian journal of medical and biological research, 27(5), 1994, pp. 1129-1134
Exogenous Ca2+ at concentrations up to 3.5 mM increases the sucrose-in
duced acidification of the culture medium when the mold Neurospora cra
ssa is grown on low-phosphate (Pi) medium at pH 7.8. Induction depends
on the pH of the culture medium adjusted for conidial inoculation and
on the absence of carbon sources generating cytoplasmic acetyl CoA. F
urthermore, the excretion of Pi-repressible acid and alkaline phosphat
ases was not stimulated by increasing exogenous Ca2+ levels. We also p
rovide evidence that the extracellular pH monitoring by Neurospora cra
ssa may be a determinant in the selective excretion of Pi-repressible
acid and alkaline phosphatases.