EFFICIENT IDENTIFICATION AND REGIONAL POSITIONING OF YAC AND COSMID CLONES TO HUMAN CHROMOSOME-21 BY RADIATION FUSION HYBRIDS

Authors
KUMLIEN J LABELLA T ZEHETNER G VATCHEVA R NIZETIC D LEHRACH H
Citation
J. Kumlien et al., EFFICIENT IDENTIFICATION AND REGIONAL POSITIONING OF YAC AND COSMID CLONES TO HUMAN CHROMOSOME-21 BY RADIATION FUSION HYBRIDS, Mammalian genome, 5(6), 1994, pp. 365-371
Citations number
28
Categorie Soggetti
Biology,"Genetics & Heredity","Biothechnology & Applied Migrobiology
Journal title
Mammalian genome → ACNP
ISSN journal
09388990
Volume
5
Issue
6
Year of publication
1994
Pages
365 - 371
Database
ISI
SICI code
0938-8990(1994)5:6<365:EIARPO>2.0.ZU;2-V
Abstract
The use of integrated mapping strategies involving bacterial, yeast, a nd rodent cells as hosts simplifies the construction of maps, which co mbine long-range order, high resolution, and easy access to the cloned DNA. Radiation-fusion hybrids offer a specially powerful long-range m apping system for human chromosomes. We describe here techniques for e stablishing a radiation-fusion hybrid map of Chromosome (Chr) 21q and its integration with local information on YAC and cosmid positions.