A NEW MATRIX FOR AFFINITY-CHROMATOGRAPHY AND ITS APPLICATION IN THE SEPARATION OF A HUMAN MONOCLONAL-ANTIBODY

Authors
GUO W SHANG ZH YU YN ZHOU LM
Citation
W. Guo et al., A NEW MATRIX FOR AFFINITY-CHROMATOGRAPHY AND ITS APPLICATION IN THE SEPARATION OF A HUMAN MONOCLONAL-ANTIBODY, BMC. Biomedical chromatography, 8(3), 1994, pp. 142-144
Citations number
6
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy",Biology
Journal title
BMC. Biomedical chromatography → ACNP
ISSN journal
02693879
Volume
8
Issue
3
Year of publication
1994
Pages
142 - 144
Database
ISI
SICI code
0269-3879(1994)8:3<142:ANMFAA>2.0.ZU;2-I
Abstract
A method for the preparation of a p-aminobenzene sulphonyl ethyl conta ining crosslinked Sepharose 4B (ABSE-Sepharose 4B-CL) is described. tr ypsin, bovine serum albumin (BSA) and concanavalin A (Con A) were immo bilized onto this matrix by diazotization. Conditions for the coupling reaction were investigated. The activity of immobilized trypsin reach ed 1.4 X 10(4) U/g, and 25 mg BSA can be coupled onto 1 g ABSE-Sepharo se 4B-CL under the optimal conditions. An affinity medium with immobil ized Con A as ligand was prepared by this method, and was used in the separation of a human monoclonal antibody.