GYRB MUTATIONS IN COUMERMYCIN A(1)-RESISTANT BORRELIA-BURGDORFERI

Authors
SAMUELS DS MARCONI RT HUANG WM GARON CF
Citation
Ds. Samuels et al., GYRB MUTATIONS IN COUMERMYCIN A(1)-RESISTANT BORRELIA-BURGDORFERI, Journal of bacteriology, 176(10), 1994, pp. 3072-3075
Citations number
42
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
176
Issue
10
Year of publication
1994
Pages
3072 - 3075
Database
ISI
SICI code
0021-9193(1994)176:10<3072:GMICAB>2.0.ZU;2-4
Abstract
We have isolated and characterized mutants of Borrelia burgdorferi tha t are resistant to the antibiotic coumermycinn A(1) which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher re sistance to coumermycin A(1) than wild-type B. burgdorferi. In each ca se, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DN A gyrase are also associated with resistance to coumarin antimicrobial agents.