ETHIDIUM-BROMIDE INTERCALATION AND CHROMATIN STRUCTURE - A SPECTROPOLARIMETRIC ANALYSIS

Authors
VERGANI L GAVAZZO P MASCETTI G NICOLINI C
Citation
L. Vergani et al., ETHIDIUM-BROMIDE INTERCALATION AND CHROMATIN STRUCTURE - A SPECTROPOLARIMETRIC ANALYSIS, Biochemistry, 33(21), 1994, pp. 6578-6585
Citations number
42
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062960
Volume
33
Issue
21
Year of publication
1994
Pages
6578 - 6585
Database
ISI
SICI code
0006-2960(1994)33:21<6578:EIACS->2.0.ZU;2-E
Abstract
Native chromatin has been characterized at different ionic strengths, according to different methods of preparation, by means of circular di chroism and ethidium bromide intercalation. For a more precise interpr etation of these results, a series of analogous experiments on phagic DNA, as a function of its circularization, and on mononucleosomes have been performed. Results are discussed here in terms of high order chr omatin structure, DNA supercoiling, and topological constraints.