The phospholipidic classes of the natural pulmonary surfactant Curosur
f(R) were separated by bidimensional TLC and isolated. Quantitative an
alysis of each class was made by colorimetric determination of phospho
rus. FD/MS and NH3-CI/MS were used to identify individual components.
GC was used for qualitative/quantitative analysis of fatty acid moieti
es as methyl esters, using internal standards, by comparison with auth
entic samples.